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ID 16889
Eprint ID
16889
FullText URL
Thumnail 94_829.pdf 653 KB
Title Alternative
Immunological studies on the etiology of gingival hyperplasia induced by chronic administration of Phenytoin I. Phenytoin radioimmunoassay
Author
Uokawa, Masayoshi
Abstract
The present experiment was initiated in order to study the immunological aspects of the etiology of gingival hyperplasia. To confirm that Phenytoin (Ph) it self can provoke an immune response as partial immunogen in the animal body, the least modified compound of Ph (p-aminophenytoin) was used for bridging with carrier protein (BSA). Rabbits were immunized initially with BSA-Ph in Freund's complete adjuvant and booster injections were carried out with BSA-Ph in Freund's incomplete adjuvant. The radio-immunoassay was carried out by competing unlabelled Ph with a known amount of 3H Ph, and B/T % was measured. The assay can detect as little as 2 μg of Ph. The specificity reaction of antisera with Ph metabolite or the other anticonvulsants revealed that antisera reacted with HPPH(major metabolite of Ph) very slightly but not at all with other anticonvulsants. Parallel analysis performed with HPLC with the same sample revealed excellent correlation between the two methods. The method here described is very simple and provides high accuracy. The success of anti-Ph antisera formation with antigen which provides unmodified Ph as the hapten seems to ensure that BSA-Ph conjugate can be used as a suitable immunogen in provocation of delayed hypersensitivity of Ph.
Keywords
Phenytoin
Radioimmunoassay
HPLC
Published Date
1982-10-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume94
Issue
issue9-10
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
829
End Page
842
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/94/9-10/94_9-10_829/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma