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ID 16857
Eprint ID
16857
FullText URL
Thumnail 94_729.pdf 1.43 MB
Title Alternative
An analysis of nucleoproteins of replicating and nonreplicating simian virus 40
Author
Irisawa, Minoru
Nakamura, Takashi
Watanabe, Sekiko
Oda, Takuzo
Abstract
Simian virus 40 (SV40) chromatin and mature virion were extracted from formaldehyde-fixed SV40-infected CV-1 cells. Mature virion was eliminated from SV40 chromatin by sucrose density gradient centrifugation. Replicating and nonreplicating SV40 chromatin were separated on CsCl/ guanidine-HCl density gradients. Replicating SV40 chromatin was lower in density than the nonreplicating form. The analysis of chromatin proteins by polyacrylamide gel electrophoresis and silver staining revealed that replicating chromatin has less H1 histone than the nonreplicating form.
Keywords
SV40
replication
Published Date
1982-08-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume94
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
729
End Page
734
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/94/7-8/94_7-8_729/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma