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ID 16786
Eprint ID
16786
FullText URL
Thumnail 94_439.pdf 892 KB
Title Alternative
Studies on acatalasemia Part 1. An advanced screening method for hypocatalasemia, using perborate as substrate, with special reference to an experimental -semiautomatic- device.
Author
Matsuda, Seiichi
Abstract
An advanced screening method of hypocatalasemia was designd, using (1) perborate as substrate (perborate method) which is stabler and easier to prepare than hydrogen peroxide (H(2)O(2)) as substrate (H(2)O(2) method), and (2) an experimental device which makes the reaction procedure semi-automatic. The following results were obtained; 1. Regression equation was established between the catalase activities measured by perborate method and those of H(2)O(2) method. 2. Discriminant point was calculated from this equation with the discriminant value by H(2)O(2) method established by Ogata et al. The volume of potassium permanganate to be added for the final discrimination, thereby, was calculated. 3. The reaction procedure could be made semi-automatic by an experimental device which consists of a circulating water-bath and a rotary type fraction collecter. Eighteen specimens could be examined in about 10 minutes. 4. In borderline cases quantitative analysis is immediately available with this device.
Keywords
acatalasemia
perborate
screening
semi-automatic
Published Date
1982-06-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume94
Issue
issue5-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
439
End Page
445
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/94/5-6/94_5-6_439/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma