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ID 16504
Eprint ID
16504
FullText URL
Thumnail 96_815.pdf 5.68 MB
Title Alternative
Studies on DNA polymerases involved in repair (unscheduled) DNA synthesis in cultured human cells treated with carcinogens, such as MNNG and 4NQO
Author
Hosogi, Nobuo
Abstract
DNA polymerases involved in carcinogen-induced repair (unscheduled) DNA synthesis in HeLa and HEp-2 cells were studied by using selective inhibitors (aphidicolin, 2', 3'-dideoxythymidine-5'-triphosphate and N-ethylmaleimide) for DNA polymerases. DNA synthesis was analyzed biochemically and autoradiographically in intact cells and in permeable cells. To measure repair DNA synthesis with minimum interference of replicative DNA synthesis, carcinogens were used at the concentration inducing maximum repair DNA synthesis with each drug and also repair DNA synthesis was measured in the presence of 10 mM hydroxyurea which preferentially inhibits replicative DNA synthesis. The maximum induction of repair DNA synthesis in HeLa cells in the present assay conditions was obtained at 100-200 μg/ml (0.68-1.4 mM) of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), 10-50 μM of 4-nitroquinoline-1-oxide (4NQO), 0.5-1 mM of methyl methanesulfonate and approximately 1mM of N-methyl-N-nitrosourea (MNU). Carcinogen-induced repair DNA synthesis was partially inhibited by a polymerase α-specific inhibitor, aphidicolin. The aphidicolin-resistant unscheduled DNA synthesis in permeable cells was sensitive to 2', 3'-dideoxythymidine-5'-triphosphate which preferentially inhibits DNA polymerases β and γ. The present results indicated that both aphidicolin-sensitive and -insensitive DNA polymerases, e.g. α-DNA polymerase and a non-α-DNA polymerase (possibly DNA polymerase β) were involved in repair DNA synthesis induced by these carcinogens in human cells. The participation of α- and non-α-DNA polymerases in repair DNA synthesis was suggested to differ depending on cell types and carcinogens used for the induction of repair DNA synthesis.
Keywords
癌原性化学物質
修復DNA合成
DNAポリメラーゼ
培養ヒト細胞
aphidicolin
MNNG
4NQO
Published Date
1984-08-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume96
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
815
End Page
827
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/96/7-8/96_7-8_815/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma