このエントリーをはてなブックマークに追加
ID 16453
Eprint ID
16453
FullText URL
Thumnail 96_641.pdf 1.76 MB
Title Alternative
Studies on determination of urinary protein Part Ⅳ. Identification of Donaggio reaction positive substances and perchloric acid soluble protein in urine
Author
Tanaka, Yukiko
Abstract
It is well known that serum albumin, γ-G-globulin and glycoprotein increase in urine after physical load. There are two ways, the Donaggio reaction and ASPRO method (a new acid soluble glycoprotein assay using coomassie brilliant blue G-250), to measure glycoprotein and other acid soluble protein for estimating the physical load. This investigation was performed to identify the composition of protein detected by the Donaggio reaction (DRPSu) and of perchloric acid soluble protein detected by the ASPRO method in urine (ASPROu). In DRPSu, albumin, α1-acidglycoprotein, α2-HS-glycoprotein, Zn-α2-glycoprotein, α1-antitrypsin, α1-antichymotrypsin and transferrin were recognized by double diffusion, single radial immunodiffusion and immunoelectrophoresis. In ASPROu, the same proteins as above were recognized according to the same immunological methods. However, a different ratio of protein components was recognized between DRPSu and ASPROu by SDS-PAGE. The perchloric acid soluble protein in urine before and after exercise from high school boys was measured by the ASPRO method, and a significant difference was recognized between before and after exercise.
Keywords
Donaggio 反応陽性物
urinary protein
ASPRO法
SDS-PAGE
Published Date
1984-04-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume96
Issue
issue5-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
641
End Page
649
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/96/5-6/96_5-6_641/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma