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ID 16447
Eprint ID
16447
FullText URL
Thumnail 96_615.pdf 385 KB
Title Alternative
Studies on antibody-anticancer drug conjugate. Part 1. Production of anti-Ia monoclonal antibody-Neocarzinostatin conjugate and its in vitro activity.
Author
Okazaki, Morihiro
Abstract
A murine IgM monoclonal antibody specific for human Ia antigen was purified by Sephacryl S-300 chromatography from ascites obtained from BALB/c mice inoculated with the antibody-secreting hybridoma. The anti-Ia antibody (H-2) was reacted with Neocarzinostatin (NCS) in the presence of carbodiimide. The resulting mixture was chromatographed on a Sephacryl S-300 column. The first fraction was shown to contain H-2-NCS conjugate, but not free NCS, by the elution profile, as well as by the Sarcina lutea growth inhibition assay. A membrane immunofluorescence test with anti-NCS and anti-mouse immunogloblin demonstrated specific localization of H-2-NCS on the cell surface of Ia-bearing NALL-1 cells. No localization of NCS was shown when the cells were incubated with free NCS. H-2-NCS inhibited the growth and (3)H-TdR incorporation of NALL-1 cells 100 times more potently than free NCS alone, when the cells were reacted with the drugs at 4℃ for 30 min. and culutured further for 14 hrs. These results indicate that the H-2-NCS cnojugate retained both NCS and antibody activities, and exerted antibody-targeting NCS cytotoxicity in vitro. Thus the conjugate should be useful for cancer immunochemotherapy.
Keywords
免疫化学療法
抗体結合抗癌剤
単クローン抗体
Neocarzinostatin
Published Date
1984-04-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume96
Issue
issue5-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
615
End Page
621
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/96/5-6/96_5-6_615/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma