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ID 16402
Eprint ID
16402
FullText URL
Thumnail 95_1087.pdf 2.14 MB
Title Alternative
Basic study of the horseradish peroxidase method II. Electron microscopic study
Author
Ishimura, Hitoshi
Abstract
Rat sciatic nerve was observed by electron microscopy using the horseradish peroxidase (HRP) method. The nerve was crushed with a needle and HRP was injected around a sciatic nerve. After 5 hours to 3days, the nerve and spinal cord were dissected and observed under an electron microscope. Five hours after the injection, HRP granules were identified in the axoplasm of myelinated nerve fibers at the injection site. Many HRP granules were distributed in the cytoplasm of motor neurous of the spinal cord 3 days after the injection of HRP. There was no change in cell organella. The neurons in the spinal ganglia also did not show any ultrastructual changes in cell organella 2 to 3 days after the injection of HRP and had many HRP granules in their cytoplasm. These results showed that HRP injected into the crushed end of a sciatic nerve did not induce morphological changes in the motor neurons of the spinal cord and, sensory neurons in spinal ganglia.
Keywords
HRP法
坐骨神経
脊髄前角細胞
脊髄神経節
電子顕微鏡観察
Published Date
1983-10-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume95
Issue
issue9-10
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1087
End Page
1095
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/95/9-10/95_9-10_1087/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma