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ID 16294
Eprint ID
16294
FullText URL
Thumnail 95_387.pdf 873 KB
Title Alternative
Partial purification and properties of Ca(2+)-binding protein from rat liver mitochondrial matrix.
Author
Tokuda, Masaaki
Abstract
A 193-fold purification of Ca(2+)-binding protein from rat liver mitochondrial matrix was achieved. The Ca(2+)-binding protein consisted of 3 polypeptide subunits whose respective molecular weights by SDS polyacrylamide gel electrophoresis were 62K, 49K and 37K. The molecular weight of the protein was 150K to 220K. The Kd for Ca(2+) was 1.3×10-(5)M and lower for Mn(2+) and Mg(2+). The protein was inactivated by heat treatment at 100℃ for 1 min, though stable against treatment with 0.5% w/v trypsin at 37℃ for 30 min. Ruthenium red did not inhibit Ca(2+)-binding.
Keywords
肝ミトコンドリア
Ca(2+)結合蛋白質
Published Date
1983-04-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume95
Issue
issue3-4
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
387
End Page
393
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/95/3-4/95_3-4_387/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma