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ID 15963
Eprint ID
15963
FullText URL
Thumnail 100_599.pdf 590 KB
Title Alternative
Metabolic pathway of guanidinosuccinic acid biosynthesis
Author
Matsuda, Rikiya
Abstract
In order to understand the metabolic abnormalities occurring with renal failure, the biosynthetic pathway of guanidinosuccinic acid (GSA) was investigated in normal rat liver, because GSA is known as a uremic toxin and its synthetic pathway is unclear.After fresh liver was homogenized and centrifuged for 60 min at 100,000×g, the supernatant was fractionated by precipitation with ammonium sulfate. It was found that the activity of GSA biosynthetic enzymes was highest in the fraction between 30% and 40% saturation of ammonium sulfate. The reaction mixture contained potassium phosphate buffer (pH7.4), aspartate, urea, ATP and an ATP-generating system, MnCl(2), and enzyme solution. The optimum pH was 7.4. The activity was strongly accelerated by Mn(++) and Cu(++), while it was completely inhibited by Fe(++). Hydroxyurea, L-canaline, L-canavanine, and L-arginine could not substitute for urea.These results suggest that GSA is not formed by the transamidination of arginine to aspartate as proposed by Cohen, or by way of the guanidine cycles proposed by Natelson. It is possible that GSA is mainly synthesized from urea and aspartate directly in rat liver.
Keywords
guanidinosuccinic acid
enzyme activity
metabolic pathway
urea
Guanidine Cycle
Published Date
1988
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume100
Issue
issue5-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
599
End Page
608
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/100/5-6/100_5-6_599/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma