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ID 15951
Eprint ID
15951
FullText URL
Thumnail 99_737.pdf 9.9 MB
Title Alternative
Morphological and functional analysis of basophils in chronic myelocytic leukemia (CML) II. Scanning electron microscopic observations of basophils did examination of IgE receptors and histamine content.
Author
Fujimoto, Shuitsu
Abstract
To clarify the ultrastructural and functional charcteristics of basophils in CML, the author observed the morphological changes of cells by scanning electron microscopy (SEM), and examined the density and redistribution of IgE receptors using an immuno-SEM technique. Histamine content and release of histamine from basophils upon addition of anti-IgE were also measured. Mature basophils, corresponding to types I and II in the basophilogram, had a spherical shape, while immature cells, corresponding to types III and IV, were pearshaped. Type I, II, III and IV basophils differed in the number of microvilli, more mature basophils having more microvilli than immature basophils. The mean number of bound immunolatex particles, which indicate IgE receptors, was 19.3±14.0/basophil in healthy subjects, 42.4±30.9 in bronchial asthmatic, and 3.7±6.6 in CML patients. The number of IgE receptors in the blastic phase of CML was much lower than in the chronic phase of CML. The histamine content of basophils was 0.5±0.3 pg/cell in the blastic phase of CML compared with 1.4±0.4 pg/cell in the chronic phase of CML, 1.3±0.4 pg/cell in healthy subjects and 1.4±0.4 pg/cell in bronchial asthmatic. These data indicate that immature basophils of CML have some morphological and functional defects.
Keywords
chronic myelocytic leukemia
basophils
IgE receptor
histamine
immuno SEM
Published Date
1987-08-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume99
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
737
End Page
749
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/99/7-8/99_7-8_737/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma