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ID 15948
Eprint ID
15948
FullText URL
Thumnail 100_533.pdf 2.72 MB
Title Alternative
Effect of dantrolene on rat brain tryptophan hydroxylase
Author
Okada, Shoshiro
Abstract
Recently, dantrolene has been applied to the treatment of neuroleptic malignant syndrome. In some treated patients, dramatic changes in the electroencephalogram and in the levels of monoamine metabolites in cerebrospinal fluid were observed. Nonetheless, little is known about the effect of dantrolene on the central nervous system. In this study, I investigated the effects of dantrolene on rat brain tryptophan hydroxylase (TrpOHase) activity. Intraperitoneal administration of dantrolene (20mg/kg) increased the TrpOHase activity to approximately 130% of the control level. An increase in TrpOHase activity was found in a synaptosomal fraction. Activation of TrpOHase by dantrolene was observed in a dose dependent manner in vitro, and the highest activity was obtained with 6.0 μM dantrolene (approximately 125% of the control level). Preincubation with dantrolene had no remarkable effect on TrpOHase activity. The effect of dantrolene on TrpOHase was manifested kinetically as a decrease in the apparent Km of the enzyme for cofactors (BH(4) and 6MPH(4)).Dantrolene had no effect on TrpOHase partially purified by affinity chromatography. These findings suggest that dantrolene influences the central nervous system and alters TrpOHase activity.
Keywords
tryptophan hydroxylase
dantrolene
悪性症候群
中枢神経系
Published Date
1988
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume100
Issue
issue5-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
533
End Page
545
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/browse/joma/100/5-6/_contents/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma