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ID 15864
Eprint ID
15864
FullText URL
99_125.pdf 2.73 MB
Title Alternative
Immunochemical Studies of Islet Cell Cytoplasmic Antibodies (ICA) and Characteristics of Their Target Antigens
Author
Takahashi, Kenji
Abstract
In the present study, the antigen proteins of Sprague-Dawley rat pancreas islets recognized by islet cell cytoplasmic antibodies (ICA) cross-reactive with rat islets were characterized. A detergent lysate of rat islets (islet lysate) and Maclura pomifera agglutinin-binding glycoprotein (MPA-GP) of rat pancreas were used as antigen sources. Reactivities of these two crude antigen proteins against 4 ICA-positive sera from patients with Type 1 (insulin-dependent) diabetes mellitus (IDDM) were examined by SDS-polyacrylamide gel electrophoresis followed by immunoblotting, and by fluoroimmunoassay (FIA). Two of the 4 ICA-positive sera specifically bound a peptide of 59kdalton (Kd) from islet lysates. This peptide was detected even after absorption of sera with rat acetone liver powder or viable rat islet cells. One serum sample was examined for rectivities to MPA-GP, and the serum bound a peptide of 59Kd from MPA-GP. In an absorption test of the serum with MPA-GP, decreased specific fluorescence of rat pancreas islets was shown by an indirect immunofluorescent technique. The binding activity of MPA-GP to ICA-positive sera measured by the FIA method was higher in the ICA-positive sera than in the ICA-negative sera. It was concluded that a peptide of 59Kd is one of the target antigen proteins of ICA, and that the peptide has binding activity with MPA. It was suggested that ICA can be measured by the FIA method using MPA-GP. It was also suggested that ICA in sera from Type 1 diabetic patients is heterogenous and that the target antigen is polyvalent.
Keywords
islet cell cytoplasmic antibodies (ICA)
islet cell antigens
plant lectin
Maclura pomifera agglutinin
immunoblotting
Published Date
1987-02-28
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume99
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
125
End Page
134
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/99/1-2/99_1-2_125/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma