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ID 15858
Eprint ID
15858
FullText URL
Thumnail 99_85.pdf 1.76 MB
Title Alternative
Changes in guanidino compound levels in the cerebrospinal fluid of rabbits with pentylenetetrazol-induced convulsions
Author
Fujikawa, Nobumasa
Abstract
A method for repeated sampling of cerebrospinal fluid (CSF) from freely moving rabbits was developed. Levels of guanidino compounds were estimated in the CSF of rabbits with pentylenetetetrazol (PTZ)-induced convulsions by high performance liquid chromatography. A catheter was implanted via the skull into the cisterna magna and was fixed by screws. One week after the operation, 100μl of CSF was collected with a microsyringe. PTZ solution (3.3 %) was injected intraveneously, and CSF was collected simultaneously until clonic-tonic convulsions appeared. Clonic-tonic convulsions continued for about 20 seconds. After that, no convulsion was induced for one hour after the injection. CSF was also collected 20 min and 60 min after the injection. For the control, CSF was collected 5 min before PTZ injection. Guanidinoacetic acid, creatinine and arginine were found in 100μl of rabbit CSF. Levels of guanidinoacetic acid and creatinine increased and then recovered to the control level 2 hours after convulsions. Arginine started to decrease 2 hours after convulsions and continued to decrease gradually untill 2 days after convulsions. The levels recovered to the control level 4 days after convulsions.
Keywords
髄液採取方法
PTZ
ウサギ
guanidino化合物
guanidinoacetic acid
Published Date
1987-02-28
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume99
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
85
End Page
96
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/99/1-2/99_1-2_85/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma