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ID 15191
Eprint ID
15191
FullText URL
Title Alternative
Antifungal effect of "Mokusaku-Eki" against Trichophyton mentagrophytes and Trichophyton rubrum
Author
Watanabe, Kumi
Sumiyoshi, Kazuko
Kaneko, Noriyo
Kawata, Chieko
Abstract
足白癬は糖尿病患者で合併しやすく,高齢者にも多発する。近年,白癬菌に対する木酢液の抗菌作用が知られており,この最適有効濃度を明らかにするため本研究を企画した。白癬菌はTrichophyton mentagrophytesとTrichophyton rubrumの2菌種を用い,木酢液は市販品を用いた。発育抑制テストは終濃度2,3,4,5%の木酢液を含んだ寒天平板希釈法で行った。殺菌テストは2.5,5,10%溶液を胞子と接触後,寒天平板培地に接種し,菌の発育の有無を観察した。発育抑制作用は,両菌種とも3%以上で7日培養後の菌糸の発育は見られなかった。殺菌作用は, T. mentagrophytesでは10%,5%においては6時間接触で,2.5%においては24時間接触で菌糸の発育が見られず, T. rubrumでは,10%以上においては24時間接触で,5%においては48時間接触で菌糸の発育が見られなかった。足浴等の看護ケアに木酢液を応用するためには,今後より短時間の接触で効果が得られる方法の検討が必要である。
Abstract Alternative
The Mokusaku-Eki is a kind of pyroligneous acid and a dark brown colored solution which obtained from the charcoal burner of Quercus spp. woods as a by-product. In Japan, the antifungal effects of this solution are well known in public. However, it is not clear that minimal inhibitory concentration (MIC) of Mokusaku-Eki against Trichophyton spp. In this study, we examined their antifungal activities in view of MIC and germicidal effect. To examine MIC of Mokusaku-Eki against Trichophyton spp, agar, dilution assay were used. Sabouraud agar plates containing 2%, 3%, 4%, or 5% of Mokusaku-Eki were prepared. Then the spores of T. mentagrophytes and T. rubrum inoculated onto each agar plate respectively and incubate the plates at 30℃. The growth of T. mentagrophytes and T. rubrum were completely inhibited on the agar plates contained more than 3% Mokusaku-Eki. Furthermore, we investigated the fungicidal effect of Mokusaku-Eki against these fungus. We exposed the spores of T. mentagrophytes and T. rubrum respectively to 2.5%, 4% or 10% Mokusaku-Eki for 20 min, 1 hr, 6 hr, 24 hr, and 48 hr, and then inoculated them onto Sabouraud agar plates. Exposures of the spores to 5% of the Mokusaku-Eki for 24 hr and 48 hr completely killed them and we could show that Mokusaku-Eki have also germicidation activity against both T. mentagrophytes and T. rubrum.
Keywords
木酢液 (Mokusaku-Eki)
白癬菌 (Trichophyton)
発育抑制作用 (Antifungal effect)
足部白癬 (Trichophytosis)
Published Date
2004-03-31
Publication Title
岡山大学医学部保健学科紀要
Publication Title Alternative
Bulletin of Faculty of Health Sciences Okayama University Medical School
Volume
volume14
Issue
issue2
Publisher
岡山大学医学部保健学科
Publisher Alternative
Faculty of Health Sciences Okayama University Medical School
Start Page
129
End Page
133
ISSN
1345-0948
NCID
AA11403004
Content Type
Departmental Bulletin Paper
language
日本語
OAI-PMH Set
岡山大学
File Version
publisher
Refereed
False
Sort Key
3
Eprints Journal Name
fhs