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ID 14706
Eprint ID
14706
FullText URL
Thumnail 102_199.pdf 6.1 MB
Title Alternative
Structural protein analysis of intracisternal A particles in adenovirus-induced mouse tumor
Author
Hirakawa, Eiichiro
Abstract
Analysis and purfication of structural proteins of intracisternal A particles produced in adenovirus-induced tumor were described. SDS-PAGE of purified intracisternal A particles demonstrated its major structural components, K92, K70 and K55, and minor components, K43 and K37. Two dimensional gel electrophoresis indicated a pI of K70 and K55 at 6.5 and 6.3, respectively, in the presence of sodium dodecyl sulfate. Purification of the main band, K70, in SDS-PAGE using reversed phase-HPLC was difficult in the standard acidic condition, but could be achieved in the neuttral condition. Although purificatin of K70 is generally difficult because of its hydrophobicity, the method shown here will be useful for furthey study of structural proteins of intracisternal A particles.
Keywords
小胞体内 A 粒子
アデノウイルス誘発腫瘍
構成蛋白分析
reversed phase-HPLC
Published Date
1990-02
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume102
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
199
End Page
207
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/102/1-2/102_1-2_199/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma