ID | 14604 |
Eprint ID | 14604
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FullText URL | |
Author |
Yuhara, Atsuyoshi
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Abstract | 1. The rabbits of a commercial stock were immunized by the intravenous inoculation of live Cryptococcus neoformans of the Ishino strain at the concentration of 1 mg per cc. The antigen was injected thrice a week for 3 consecutive weeks. The immune serum with the agglutination titer of 1:1, 024 against the homologous organism was obtained 5 weeks after the last inoculation.
2. The tube agglutination test with this immune serum gave the following agglutination titers, 1:128 against Cryptococcus neoformans (C2-4 strain), 1:32 against Cryptococcus diffluens and 1:8 against Torulopsis sake and Torulopsis pinus, respectively.
3. The slide agglutination test showed that the differentiation between Cryptococcus species and Torulopsis species was made if the antiserum was diluted to 1:10.
4. The γ-globulin concentation of the fluorescein isothiocyanate-labeled antibody solution was 0.52% which was completely able to differentiate between Torulopsis pinus and Cryptococcus species if diluted to 1:8. The fluorescent antibody technic was able to afford us making a diagnosis without difficulty when the fluorescein isothiocyanate-labeled antibody was prepared from the immune serum with agglutination titer as high as possible and diluting it to some extent. (In the author's experiment, diluting to 0.0165% of γ-globulin was most effective.)
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Note | 学位番号 乙第174号
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Published Date | 1966-05-30
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Publication Title |
岡山医学会雑誌
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Publication Title Alternative | Journal of Okayama Medical Association
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Volume | volume78
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Issue | issue4-5
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Publisher | 岡山医学会
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Publisher Alternative | Okayama Medical Association
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Start Page | 477
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End Page | 482
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ISSN | 0030-1558
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NCID | AN00032489
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Content Type |
Journal Article
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Official Url | https://www.jstage.jst.go.jp/article/joma1947/78/4-5/78_4-5_477/_article/-char/ja/
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Related Url | http://eprints.lib.okayama-u.ac.jp/5523/
http://eprints.lib.okayama-u.ac.jp/14601/
http://eprints.lib.okayama-u.ac.jp/14606/
http://eprints.lib.okayama-u.ac.jp/14608/
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language |
Japanese
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Copyright Holders | 岡山医学会
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File Version | publisher
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Refereed |
True
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Eprints Journal Name | joma
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