このエントリーをはてなブックマークに追加
ID 14562
Eprint ID
14562
FullText URL
Thumnail 102_143.pdf 7.79 MB
Title Alternative
Integration and expression of E1 region DNA in human adenovirus type 12-induced tumors
Author
Morikawa, Tomoko
Abstract
Integration and expression of human adenovirus type 12 (Ad12) were studied on two Ad12-induced tumor lines of C3Hf/OK mouse origin (IC and D tumors). Southern hybridization using whole Ad12 genome as probe demonstrated that DNA of IC and D tumors contained about 9 and 23 copies, respectively, of the entire Ad12 genome eqivalent per diploid cell. Left ends of Ad12 DNA appeared as off-size bands, indicating their linkage to different cellular DNAs. Southern hybridization using and Ad12 EcoRI-C fragment containing the E1 region as a probe revealed that intergration sites were much fewer than presumen numbers of integrated Ad12 genones. These results suggested that the viral and cellular DNA complex was repeated at integration sites. Northen hybridization using an Ad12 EcoRI-C fragment as a probe showed that tha E1 region was transcribed in both IC and D tumors. Much more intensive expression in the D tumor suggested possible dependence of the expression of the E1 region on the copy numbers of Ad12 genomes.
Keywords
ヒトアデノウィルス12型
E1領域
C3Hf/OK マウス
ウィルス発癌
Published Date
1990-02
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume102
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
143
End Page
152
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/102/1-2/102_1-2_143/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma