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ID 14207
Eprint ID
14207
FullText URL
Thumnail 103_823.pdf 6.73 MB
Title Alternative
Medico-legal studies on the sex-identification of biological materials by DNA analysis
Author
Nakai, Miyoko
Abstract
Sex identification of human blood, bloodstains, saliva, hair roots and bone marrow was conducted by DNA analysis. The effects of various contaminants in samples on sex identification were studied, using 32-week-old contaminated bloodstains. The effect of heat or decomposition on sex identification was examined by using the blood obtained from autopsy-cases. A 2.12 kb band with male DNA, digested with Hae Ⅲ and electrophoresed, was observed by Southern hybridization using a human Y chromosome-specific probe, while a faint 2.00 kb band with female DNA was also sometimes detected. The male 2.12 kb band was detected even in 5-year-old bloodstains. The 2.12 kb band was also detected frequently in contaminated bloodstains as old as 32 weeks. The band was observed by Southern hybridization analysis in decomposed blood as old as 7 days, while it could not be detected in heated and decomposed blood after 3 days.
Keywords
sex identification
deoxyribonucleic acid (DNA)
forensic science
bloodstains
Note
原著
Published Date
1991-08
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume103
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
823
End Page
831
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/103/7-8/103_7-8_823/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma