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ID 14171
Eprint ID
14171
FullText URL
Thumnail 103_779.pdf 611 KB
Title Alternative
Studies on the regulation of eosinophils in bronchial asthma Part 1. Characterization of eosinophil chemotactic factor (ECF) derived from peripheral blood mononuclear cells stimulated with Candida antigen
Author
Takahashi, Hisaho
Abstract
Eosinophilic infiltration in bronchial tissue is characteristic in the pathogenesis of bronchial asthma. The eosinophil chemotactic factor (ECF) derived from mononuclear cells has been reported to have some effect on the cell infiltration, and interleukin-5 (IL-5), a lymphokine derived from T lymphocytes, to be a factor related to growth, chemotaxis and activation for eosinophils. Lymphocytes accumulated in the bronchoalveolar lavage fluids of non-atopic and severe asthmatics have been shown to be highly responsive to Candida antigen, and high ECF production was observed in non-atopic and severe asthmatics by measurement of ECF activity in the supernatant of peripheral blood mononuclear cells cultured with Candida antigen. In this report, the molecular weight by gel filtration and inhibition test using anti-murine IL-5 antibody were studied to characterize the lymphocyte-derived ECF. Gel filtration analysis of the ECF indicated a molecular weight of 20,000 to 65,000 Da with a peak of activity around 40,000 to 50,000 Da. The ECF activity was reduced by incubation with anti-murine IL-5 antibody, which suggests that the supernatant contains IL-5. ECF from mononuclear cells, containing IL-5, may play an important role in the pathogenesis of eosinophil infiltration in non-atopic and severe asthmatics.
Keywords
Bronchial asthma
Candida antigen Eosinophil chemotactic factor
Interleukin-5
Note
原著
Published Date
1991-08
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume103
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
779
End Page
789
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/103/7-8/103_7-8_779/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma