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ID 14142
Eprint ID
14142
FullText URL
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Title Alternative
Analysis on the mechanism of the temperature-sensitive melibiose metabolism in Lactobacillus plantarum
Author
Tamura, Chiyuki
Abstract
L. plantarum can grow on melibiose when incubated at 30℃ but not when incubated at 37℃. In this study, the temperature sensitivity of melibiose metabolism was analyzed. The melibiose metabolism seemed to consist of the melibiose transport system and hydrolyzing enzyme, α-galactosidase. α-Galactosidase was induced with melibiose at 30℃ but not at 37℃. This enzyme induced at 30℃ hydrolyzed colorimetric substrate, α-ONPG at 30℃ and 37℃. On the other hand, lactose induced α-galactosidase at both temperatures. These findings suggested that the temperature sensitivity of melibiose metabolism was not due to the induction and/or function of α-galactosidase. Thus, the effect of temperature on induction and function of the melibiose transport system was examined. Cells grown on melibiose at 30℃ exhibited the rapid uptake of [(3)H] -melibiose at 30℃ and 37℃, while [(3)H] -melibiose was not taken up by the cells grown at 37℃. The induction of the melibiose transport system seemed to be temperature-sensitive because the transport system was not inactivated by exposure of the cells to 37℃ for 3 hours. In conclusion, the temperature sensitivity of melibiose metabolism was attributed to the temperature sensitivity of the induction of melibiose transport system.
Keywords
Lactobacillus plantarum
メリビオース
高温感受性
α-ガラクトシダーゼ
メリビオース輸送系
Note
原著
Published Date
1991-08
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume103
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
759
End Page
768
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/103/7-8/103_7-8_759/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
References
1) 早川邦彦:食品・医薬品としての乳酸菌の効用.微生物 (1985) 1, 41-50.
2) 大山嘉信:サイレージ調製と微生物.化学と生物 (1977) 15, 434-442.
3) Mundt JO : Lactic acid bacteria associated with raw plant food material. J Milk Food Technol (1970) 33, 550-553.
4) Beckwith J : Restoration of operon activity by suppressors. Biochim Biophys Acta (1964) 76, 162-164.
5) Pardee AB : An inducible mechanism for accumulation of melibiose in Escherichia coli. J Bacteriol (1957) 73, 376-385.
6) Prestidge LS and Pardee AB : A second permease for methyl-thio-β-D-galactoside in Escherichia coli. Biochim Biophys Acta (1965) 100, 591-593.
7) Tanaka K, Niiya S and Tsuchiya T : Melibiose transport in Escherichia coli. J Bacteriol (1980) 141, 1030-1036.
8) Bailey RW and Bourne EJ : Colour reactions given by sugars and diphenylamine-aniline spray reagents on paper chromatograms. J Chromatogr (1960) 4, 206-213.
9) Winkler HH and Wilson TH : The role of energy coupling in the transport of β-galactosides by Escherichia coli. J Biol Chem (1966) 241, 2200-2211.
10) Rotman B, Ganesan AK and Guzman R : Transport systems for galactose and galactosides in Escherichia coli. Ⅱ. Substrate and inducer specificities. J Mol Biol (1968) 36, 247-260.
11) Schmitt R : Analysis of melibiose mutants deficient in α-galactosidase and thiomethylgalactoside permease Ⅱ in Escherichia coli K-12. J Bacteriol (1968) 96, 462-471.
12) Burstein C and Kepes A : The melibiose permease system of Escherichia coli K 12. Biochimie (1985) 67, 59-67.
13) Schmitt R and Rotman B : α-Galactosidase activity in cell-free extracts of Escherichia coli. Biochem Biophys Res Commun (1966) 22, 473-479.
14) Tsuchiya T, Lopilato J and Wilson TH : Effect of litium ion on melibiose transport in Escherichia coli. J Membrane Biol (1978) 42, 45-59.
15) Hanatani M, Yazyu H, Shiota-Niiya S, Moriyama Y, Kanazawa H, Futai M and Tsuchiya T : Physical and genetic characterization of the melibiose operon and identification of the gene products in Escherichia coli. J Biol Chem (1984) 259, 1807-1812.
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma