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ID 13813
Eprint ID
13813
FullText URL
Thumnail 109_15.pdf 3.92 MB
Title Alternative
Formation of lentoid body from retinal pigment epithelium of chick embryo in floating culture
Author
Tsutsui, Yasuko
Abstract
We observed the morphologic changes of tissue fragments of retinal pigment epithelium (RPE) of chick embryos in floating culture under the presence of bacic fibroblast growth factor (bFGF). At higher concentration (10 and 200 ng/ml) of bFGF, rolled up RPE sheets grew to develop non-pigmented lumps with the structure like onions, while dissociated RPE cells did not from such structure. Histologically, the onion-like structure consisted of lens-fiber-like cells, positive for anti-crystallin antibody and anti-bFGF-receptor antibody. This lentoid structure was not formed at 1 ng/ml or lower concentration of bFGF. The trans differentiation from RPE cells to lens epithelial cells was dependent upon the concentration of bFGF as well as the proper interaction among RPE cells.
Keywords
浮遊培養
網膜色素上皮細胞
分化転換
水晶体様構造
bFGF
Note
原著
Published Date
1997-02-28
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume109
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
15
End Page
24
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/109/1-2/109_1-2_15/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
References
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12) 林 倫子:網膜色素上皮細胞の組織培養に関する研究-正常ニワトリ胚網膜色素上皮の培養細胞について-.日眼会誌 (1977) 81, 692-700.
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma