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ID 13794
Eprint ID
13794
FullText URL
Thumnail 106_51.pdf 552 KB
Title Alternative
The production of platelet activating factor (PAF) from neutrophils in bronchial asthmatics
Author
Tsuji, Mitsuaki
Abstract
It was recently suggested that chemical mediators such as leukotrienes and PAF are more important in the pathogenesis of bronchial asthma than histamine. In this study, to clarify the role of PAF in bronchial asthma, I measured PAF activity derived from human neutrophils by the platelet aggregation tachnique and evaluated this activity in relation to the clinical features of brochial asthma. Neutrophils were separated from heparinized venous blood of 34 bronchial asthmatics and 11 healthy subjects. After suspension at 6×10(6) cells/ml, the cells were treated with 5μg/ml Ca ionophore A23187 for 5 minutes. PAF was extracted from the cell suspension using chloroform, and the activity was measured with an aggregometer using rabbit platelets. PAF activaty was significantly higher (p<0.05) in asthmatics (8.3±6.9 pmol/1×10(6) cells) than in healthy subjects (4.1±3.2 pmol/1×10(6) cells), and tended to increase in steroid-independent asthmatics rather than in steroid-dependent asthma patients. However, there was no difference in PAF production between atopic and non-atopic asthmatics, or between the early and late onset asthmatics. PAF production was increased in patients with severe airway hypersensitivity. These finding suggest that PAF plays an important role in the development and exacerbation of asthma.
Keywords
気管支喘息
血小板活性化因子
好中球
Note
原著
Published Date
1994-02
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume106
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
51
End Page
60
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/106/1-2/106_1-2_51/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma