このエントリーをはてなブックマークに追加
ID 13787
Eprint ID
13787
FullText URL
Thumnail 106_39.pdf 588 KB
Title Alternative
The mechanism of human basophil activation associated with IgG receptor : Analysis of calcium mobilization in the human basophilic
Author
Kawata, Noriko
Abstract
To clarify the mechanism of human basophil activation via IgG receptors, calcium mobiliza-tion in response to IgG antibodies was analyzed in the human basophilic leukemia cell line KU812-F, using flow cytometry. KU812-F cells as well human basophils selectively expres-sed the FcγRⅡ subtype of IgG receptor. After stimulation with aggregated IgG, an obvious increase in [Ca(2+)]i was observed, but the increase was completely inhibited by Ⅳ. 3 (antiFcγRⅡmAb). Moreover, Ⅳ. 3 elicited a[Ca(2+)]i rise only when cross-linked on the cell surface with anti-mouse IgG. No significant histamine release was observed after any IgG stimulation and the biologic function of the FcγRⅡ-induced [Ca(2+)]i rise remains unclar. These findings suggest that the cross-linking of FcγRⅡ in KU812-F cells induces signal transduction events and initiates cell activation with the exception of histamine release, and human basophils also may be activated in vivo by IgG antibodies.
Keywords
KU812-F
Basophil
Ca(2+) response
FcγRⅡ
IgG antibody
Note
原著
Published Date
1994-02
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume106
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
39
End Page
49
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
http://www.journalarchive.jst.go.jp/japanese/jnlabstract_ja.php?cdjournal=joma1947&cdvol=106&noissue=1-2&startpage=39
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
References
1) Kimura I, Tanizaki Y, Sato S and Takahashi K : Differences in response to anti-IgE and to anti-IgG in basophils from patients with bronchial asthma. Clin Allergy (1981) 11, 31-36.
2) 木村郁郎, 谷崎勝朗, 高橋 清 : 気管支喘息における抗免疫グロブリンに対する好塩基球の反応性についてー抗ヒトIgGに対する反応性からみた分類に可能性ー. アレルギー (1976) 25, 70-75.
3) 木村郁郎, 谷崎勝朗, 佐藤周一, 高橋 清, 上田暢男, 斉藤勝剛 : In vitro免疫下における抗塩基球の生態観察ー気管支喘息患者の抗ヒトIgGに対する反応性ー. アレルギー (1976) 25, 489-492.
4) 小林 誠 : 免疫反応下における好塩基球の形態学的研究 第2編 抗ヒト免疫グロブリによる好塩基球の形態学的変化の免疫走査電子顕微鏡観察. 岡山医誌 (1979) 91, 749-761.
5) Kimura I, Takahashi K, Soda R, Kishimoto T and Matsuoka T : Basophils immunoglobulin receptors in asthmatics under immuno-scanning electron microscopy. Clin Allergy (1985) 15, 9-15.
6) 松岡 孝 : 気管支喘息患者好塩基球の免疫グロブリンレセプターとIgG抗体の作用機序に関する研究 第1編 重症難治性喘息におけるIgG抗体の役割に関する検討. 岡山医誌 (1979) 98, 525-535.
7) Huzinga TWJ, Roos D and Von dem Borne AEGKr : Neutrophil Fcγ Receptors : A two-way bridge in the immune system. Blood (1990) 75, 1211-1214.
8) Takahashi K, Takata M, Suwaki T, Kawata N, Tanimoto Y, Soda R and Kimura I : New flow cytometric method for surface phenotyping basophils from peripheral blood. J Immunol Methods (1993) 162, 17-21.
9) Kisahi K : A new leukemic cell line with Philadelphia chromosome characterized as basophil precur-sors. Leuk Res (1985) 9, 381-390.
10) Minta A, Kao JPY and Tsien RY : Fluorescent indicatiors for cytosolic calcium based on rhodamine fluorescein chromophores. J Biol Chem (1989) 264, 8171-8178.
11) Kao JPY, Harootunian AT and Tsien RY : Photochemically generated cytosolic calcium pulses and their detection by fluo-3. J Biol Chem (1989) 264, 8179-8184.
12) Deber CM, Tom-Kun J, Mack E and Grinstiein S : Bromo-A23187 : a non fluorescent calcium ionophore for use with fluorescent probes. Anal Biochem (1985) 146, 349-352.
13) Hesketh TR, Smith GA, Moore JP, Taylor MV and Metcalfe JC : Free cytoplasmic calcium concentration and the mitogenic stimulation of lymphocytes. J Biol Chem (1983) 258, 4876-4882.
14) Vandenberghe PA and Ceuppens JL : Flow cytometric measurement of cytoplasmic free calcium in human peripheral blood T lymphocytes with flou-3, a new fluorescent calcium indicator. J Immunol Methods (1990) 127, 197-205.
15) McBride P, Bradely D and Kaliner M : Evaluation of a radioimmunoassay for histamine measure-ment in biological fluids. J Allergy Clin Immunol (1988) 82, 638-646.
16) Hirai K, Morita Y, Misaki Y, Ohta K, Takaishi T, Suzuki S, Motoyoshi K and Miyamoto T : Modulation of human basophil histamine release by hemopoietic frowth factors. J Immunol (1988) 141, 3958-3964.
17) Anselmino LM, Perussia B and Thomas L : Human basophil selectively express the Fcγ RⅡ (CDw32) subtype of IgG receptor. J Allergy Clin Immunol (1989) 84, 907-914.
18) Stain C, Stockinger H, Scharf M, Jaerer U, Goessinger H, Lechner K and Bettelheim P : Human blood basophils display a unique phenotype including activation linked membrane structures. Blood (1987) 70, 1872-1879.
19) Fukuda T, Kishi K, Ohnishi Y and Shibata A : Bipotential cell differentiation of KU-812 : evidence of a hybrid cell line that differentiates into basophils and macrophage-like cells. Blood (1987) 70, 612-619.
20) Valent P, Besemer J, Kishi K, Kaltenbrunnrer R, R, Kuhn B, Maurer D, Lechner K and Bettelheim P : IL-3 promotes basophilic differentiation of KU812 cells through high affinity binding sites. j Immunol (1990) 145, 1885-1889.
21) Kochan J, Pettine LF, Hakimi J, Kishi K and Kinet JP : Isolation of the gene coding for the alpha subunit of the human high affinity IgE receptor. Nucleic Acids Res (1988) 16, 3584.
22) Ravetch JV and Kinet JP : Fc Receptors. Annu Rev Immunol (1991) 9, 457-492.
23) Van de Winkel JGJ and Anderson CL : Biology of human immunoglobulin receptors. J Leukocyte Biol (1991) 49, 511-524.
24) Kimberly RP, Ahlstrom JW, Click ME and Edberg JC : The glycosyl phoshatidylinositol-linked FcγⅢ Rpmn mediates transmembrane signaling events distinct from FcγRⅡ. J Exp Med (1990) 171, 1239-1255.
25) Anderson GP and Anderson C : Signal transduction by the platelet Fc receptor. Blood (1990) 76, 1165-1172.
26) Parish W : Short-term anaphylactic IgG antibodies in human sera. Lancet (1970) 2, 590-592.
27) Grant JA and Lichtenstein LM : Reversed in vitro anaphhylaxis induced by anti-IgG : specificity of the reaction and comparison with antigen-induced histamine release. J Immunol (1972) 109, 20-25.
28) Nakagawa T, Stadler BM, Heiner DC, Skvaril F and DeWeck AL : Flow cytometric analysis of human basophil degraulation. Ⅱ. Degranulation induced by anti-IgE, anti-IgG 4 and the calcium ionophore A23187. Clin Allergy (1981) 11, 21-30.
29) Fagan DL, Slaughter CA, Capra JD and Sullivan TJ : Monoclonal antibodies to immunoglobulin G 4 induce histamine release from human basophils in vitro. J Allergy Clin Immunol (1982) 70, 399-404.
30) Van Toorenenbergen AW and Aalberse RC : IgG 4 and passive sensitization of basophil leukocytes. Int Arch Allergy Appl Immunol (1981) 65, 432-440.
31) Devey ME and Panzni R : The IgG subclass antibodies to castor bean allergen in patients with allergic asthma ; detection of a high incidence of antibodies of the IgG 4 subclass. Clinn Allergy (1975) 5, 353-361.
32) Ishizaka T, Sterk AR and Ishizaka K : Demonstration of Fcγ receptors on human basophil granulocytes. J Immunol (1979) 123, 578-583.
33) Lichtenstein LM, Sobotka AK, White JM and Hamilton R.G : Anti-human IgG causes basophil histamine release by acting on IgG-IgE complexes bound to IgE receptors. J Immunol (1992) 148, 3929-3936.
34) Shew RJ, Walsh GM, Cromwell O, Moqbel R, Spry CJF and Kay AB : Activated human eosinophils genrate SRS-A leukotriens follwing IgG-dependent stimulation. Nature (1985) 316, 150-152.
35) Seder RA, Paul WE, Ben-Sasson SZ, LeGros GS, Sobotka AK, Finkelman FD, Pierce JH and Plaut M : Production on interleukin-4 and other cytokines following stimulation of mast cell lines and in vivo mast cells/basophils. Int Arch Appl Immunol (1991) 94, 137-140.
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma