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ID 13770
Eprint ID
13770
FullText URL
Thumnail 108_13.pdf 2.02 MB
Title Alternative
The expansion and antitumoral activity of recombinant IL-2 activated tumor infiltrating lymphocytes from human cancers
Author
Matsumoto, Mitsuaki
Abstract
Tumor infiltrating lymohocytes (TIL) were isolated from various human cancers and cultured. The expansion, cytotoxicity, phenotype, and perforin, which is considered the cytotoxic factor of killer cells, of TIL were examinel. TIL cultured in 1000u/ml recombinant interleukin-2 (rIL-2) with 20% conditioned medium (CoM) exhibited far better expansion capability than that of TIL cultured in rIL-2 1000u/ml alone, and continued to expand up to 14 weeks. The maximal expansion index (EI) was 23912. When TIL arrested expansion during the culture, the addition of PHA restored the ability to expand. The CoM was composed of spent medium from a 3-day culture of peripheral blood mononuclear cells from cancer patients. The lymphokine-activated killer (LAK) cells induced by the culture was utilized for clinical adoptive immunotherapy in our department. Cytotoxicity against K562 and Daudi target cells was highest at 2 to 3 weeks, and disappeared between 4 and 7 weeks of culture. The phenotype of fresh TIL was mainly CD3(+) T cells, predominantly CD4(+) cells, but CD8(+) cells became predominant in long term culture. The frequency of IL-2R(+) and HLA-DR+ cells was correlated with the expansiveness of TIL. The appearance and intensity of perforin positive cells were associated with the level of cytotoxicity of cultured TIL.
Keywords
腫瘍内浸潤リンパ球
recombinant interleukin-2
細胞傷害活性
リンパ球表面抗原
パーフォリン
Note
原著
Published Date
1996-02-29
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume108
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
13
End Page
29
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/108/1-2/108_1-2_13/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma