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ID 13720
Eprint ID
13720
FullText URL
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Title Alternative
Sugar chain alterations of glycoproteins in spent culture media of human hepatocellular carcinoma cell lines analyzed by lectin-affinity electrophoresis
Author
Fukazawa, Masako
Abstract
Sugar chain alterations of α-fetoptotein (AFP),α1-Antitrypsin (AAT) and transferrin (Tf) in spent culture media of human hepatoma cell lines, HuH-7, huH-1, Hep-G2 and KIM-1, were analyzed by lectin-affinity electrophoresis with Concanavalin A (Con A), Lens culinaris agglutinin (LCA) and Phaseolus vulgaris lectin (E4-PHA) and antibody-affinty blotting. Their glycoforms demonstrated lectin-dependent characteristic patterns similar to the glycoforms of AFP in hepatocellular carcinomas, although their separation became less chear as the number of sugar chains per molecule increased. The proportions of band intensities of the glycoform of AAT and Tf corresponding to AFP-C1, AFP-L3 and AFP-P4 increased consistently in all cell lines. However, the extent of the increases in the glycoforms differed from one cell line to another, reflecting tumor heterogenity. On the other hand, the sugar chain alterations of glycoproteins were similar in each cell line, although the glycoforms separated with Con A showed slightly different patterns from those separated with LCA. Accordingly, hepatocelluler carcinoma may be detected at an early stage by analyzing the glycoforms of serum AAT and Tf by lectin-affinity electrophoresis for cases with negative AFP.
Keywords
Glycoprotein
lectin affinity electrophoresis
cultured hepatoma cell line
Note
原著
Published Date
1998-06-25
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume110
Issue
issue1-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
53
End Page
60
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/110/1-6/110_1-6_53/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
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language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma