このエントリーをはてなブックマークに追加
ID 13198
Eprint ID
13198
FullText URL
Thumnail 119_147.PDF 317 KB
Title Alternative
Characterization of the binding of botulinum type B 16S toxin to human intestinal epithelial cells
Author
Jin, Yingji
Abstract
Botulinum neurotoxin produced by Clostridium botulinum type B is a complex of 12S and 16S toxins. 12S toxin consists of a neurotoxin and a nontoxic non-HA (NTNH). The 16S toxin consists of a neurotoxin, an NTNH, and a hemagglutinin (HA). Food-borne botulism is caused by these complex toxins, which are ingested orally and absorbed from the digestive tract across the epithelial barrier lining the gut. Here we show that the type B 16S toxin, but not the 12S toxin or the neurotoxin, binds to the T84 human intestinal epithelial cell line. We also demonstrate that the HA moiety in the 16S toxin mediates the toxin binding to the cells. The carbohydrates containing a galactose moiety inhibited the binding of the 16S toxin to the T84 cells, and neuraminidase treatment of the cells increased the 16S toxin binding. The binding of the 16S toxin to the neuraminidase-treated cells was also inhibited by carbohydrates containing a galactose moiety. These results suggest that the type B 16S toxin binds to human intestinal epithelial cells via the galactose moiety in the carbohydrate chain on the cell surface.
Keywords
ボツリヌス毒素 (botulinus toxin)
Clostridium botulinum
ボツリヌス中毒 (botulism)
T84細胞 (T84 cell)
ガラクトース (galactose)
Note
原著
Published Date
2007-09-03
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume119
Issue
issue2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
147
End Page
151
ISSN
00301558
NCID
AN00032489
Content Type
Journal Article
Related Url
http://www.okayama-u.ac.jp/user/oma/
References
1) Schiavo G, et al. : Neurotoxins affecting neuroexocytosis. Physiol Rev (2000) 80, 717-766.
2) Oguma K, et al. : Structure and function of Clostridium botulinum progenitor toxin. J Toxicol Toxin Rev (1999) 18, 17-34.
3) Sakaguchi G, et al. : Structure and function of botulinum toxins ; in Bacterial Protein Toxins, London, Academic press (1984) pp 435-443.
4) Arimitsu H, et al. : Purification of fully activated Clostridium botulinum serotype B toxin for treatment of patients with dystonia. Infect Immun (2003) 71, 1599-1603.
5) Fujinaga Y, et al. : The haemagglutinin of Clostridium botulinum type C progenitor toxin plays an essential role in binding of toxin to the epithelial cells of guinea pig small intestine, leading to the efficient absorption of the toxin. Microbiology (1997) 143, 3841-3847.
6) Fujinaga Y, et al. : Molecular characterization of binding subcomponents of Clostridium botulinum type C progenitor toxin for intestinal epithelial cells and erythrocytes. Microbiology (2004) 150, 1529-1538.
7) Fujinaga Y, et al. : Identification and characterization of functional subunits of Clostridium botulinum type A progenitor toxin involved in binding to intestinal microvilli and erythrocytes. FEBS Lett (2000) 467, 179-183.
8) Kojima S, et al. : Clostridium botulinum type A progenitor toxin binds to Intestine-407 cells via N-acetyllactosamine moiety. Biochem Biophys Res Commun (2005) 331, 571-576.
9) Nishikawa A, et al. : The receptor and transporter for internalization of Clostridium botulinum type C progenitor toxin into HT-29 cells. Biochem Biophys Res Commun (2004) 319, 327-333.
10) Uotsu N, et al. : Cell internalization and traffic pathway of Clostridium botulinum type C neurotoxin in HT-29 cells. Biochim Biophys Acta (2006) 1763, 120-128.
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
DOI
Eprints Journal Name
joma