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ID 11871
Eprint ID
11871
FullText URL
Title Alternative
IgG Heavy-Chain Subclass Typing of Human Serum M-Protein -Optimal condition for the separation and the detection with high resolution of agarose gel isoelectrofocusing-
Author
Ichimura, Mitsuko
Tohge, Hiroko
Sakiyama, Junko
Nakata, Yasunari
Endo, Hiroshi
Abstract
IgG Heavy-Chain subclass typing of human serum M-protein were isoelectrofocussed (IEF) in agarose gel, and then the bands of IgG were detected with peroxidase-conjugated anti-human IgG1-4 monoclonal antibodies on the same isoelectrofocussed agarose gel plate. This IEF enzyme immunodetection patterns were composed of four to eight discrete bands (The range of pI was 6.0 to 9.0). These bands were dependent on immunofixationbands. It was very specific and clear to detect the subclass of IgG Type M-protein in human serum using this method.
Keywords
IgG Subclass
M-Protein
Isoelectrofocusing
Monoclonal Antibodies
Published Date
1994-01-31
Publication Title
岡山大学医療技術短期大学部紀要
Publication Title Alternative
Bulletin of School of Health Sciences Okayama University
Volume
volume4
Publisher
岡山大学医療技術短期大学部
Publisher Alternative
School of Health Sciences Okayama University
Start Page
47
End Page
51
ISSN
0917-4494
NCID
AN10355371
Content Type
Departmental Bulletin Paper
language
日本語
File Version
publisher
Refereed
False
Eprints Journal Name
fhs