ID | 11039 |
Eprint ID | 11039
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FullText URL | |
Title Alternative | Novel Cyclic Dipeptide Dehydrogenase and Assay Method for Activity
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Author |
Akazawa, Kazumi
Imura, Daisuke
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Abstract | The cell-free extract prepared from cells of an albonoursin-producing actinomycete Streptomyces sp. KO2388 was found to catalyze the dehydrogenation of cyclo (L-Phe-L-Leu) (CFL) to albonoursin. This is the first report for the dehydrogenation at the α,β-positions of amino acid residues. The simple method for determining the dehydrogenation activity was devised by measuring the increase in UV absorption of the reaction mixture at 317nm, λmax(ε25,400) of albonoursin, where CFL had no absorption. Phenazine methosulfate was the most active cofactor for the dehydrogenation among several hydrogen acceptors.
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Abstract Alternative | アルボノルシン生産菌、Streptomyces sp. KO2388株の無細胞抽出液が、cyclo(L-Phe-L-Leu)からアルボノルシンへの脱水素反応を触媒することを明らかにした。アミノ酸残基のα,β-位での脱水素反応を酵素レベルで明らかにしたのはこの報告が初めてである。本反応の簡便な測定法として、基質cyclo(L-Phe-L-Leu)には認められない。アルボノルシンの特異的な317nmにおける紫外吸収の増加を測定する方法を考案した。本方法を用いて数種の水素受容体を試験したところ、フェナジンメトサルフェートが最も有効であると判った。
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Keywords | albonoursin
cyclo(phe-Leu)
dehydrogenation
phenazine methosulfate
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Published Date | 1999-02
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Publication Title |
岡山大学農学部学術報告
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Publication Title Alternative | Scientific reports of the Faculty of Agriculture, Okayama University
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Volume | volume88
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Issue | issue1
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Publisher | 岡山大学農学部
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Publisher Alternative | Faculty of Agriculture,Okayama University
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Start Page | 7
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End Page | 11
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ISSN | 0474-0254
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NCID | AN00033029
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Content Type |
Departmental Bulletin Paper
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language |
Japanese
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File Version | publisher
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Refereed |
False
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Eprints Journal Name | srfa
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