リンク 詳細 ja http://ousar.lib.okayama-u.ac.jp/metadata/49675 Itano,Arao Arakawa,Satiyo http://ousar.lib.okayama-u.ac.jp/metadata/49675 http://ousar.lib.okayama-u.ac.jp/metadata/49674 Itano,Arao http://ousar.lib.okayama-u.ac.jp/metadata/49674 1928-10-04 http://ousar.lib.okayama-u.ac.jp/metadata/49673 Kondō,Mantarō Okamura,Tamotsu http://ousar.lib.okayama-u.ac.jp/metadata/49673 1928-02-20 http://ousar.lib.okayama-u.ac.jp/metadata/18094 The supernatant of the regional lymph node of mice transplanted with Ehrlich ascites tumor were fractionated on DEAE Sephadex A-50 column chromatography with linear gradient elution. The eluted peaks were examined electrophretically. They were γ, β. α and albumin. β and α fractions were effective for suppression of Ehrlich ascites cell growth. Minimum effective β fraction was 300μg/ml. The sedimentation coefficient of β fraction was 6.5 S 20 w, and that of α fraction was 7.4 S 20 w. 岡野,厖 http://ousar.lib.okayama-u.ac.jp/metadata/18094 1968-04-30 http://ousar.lib.okayama-u.ac.jp/metadata/18092 The regional lymph nodes of mice transplanted with Ehrlich ascites tumor have an inhibitory effect for cultured Ehrlich ascites tumor. The supernatant of homogenized regional lymph nodes were fractionated on Sephadex G-200 column chromatography. The elution curve was fractionated in main three peaks, and the later half of the first peak and second peak were effective for the suppression of Ehrlioh ascites cell growth. These two fractions were agreed electrophoretically protein. 岡野,厖 http://ousar.lib.okayama-u.ac.jp/metadata/18092 1968-04-30 http://ousar.lib.okayama-u.ac.jp/metadata/18090 1. There were no changes suggestive of SLE in the rabbits that had received hydralazine in daily dossage of 200mg per kilogram body weight for as long as 4 months. 2. Of the rabbits sensitized with egg white and, in addition, administered with hydralazine, many died suddenly after injections of egg white, but a few rabbits showed anemia, probably hemolytic in type. No LE cell phenomenon or antinuclear factor (ANF) was revealed in this group. 3. Slight leucopenia, albuminuria, hypergamma-globulinemia and increased IgG were found in the rabbits injected with rat anti-rabbit cell nuclei serum. Four rabbits showed nephritis, productive in form at necropsy, with deposition of γ-globulin in glomerular capil laries as revealed by the direct FAT. Weak LE cell phenomenon and ANF were positive in one rabbit. 4. It is presumable that, as in human cases, hereditary factor is impcrtant to produce the models of auto-immune diseases in animals. 宮脇,昌 http://ousar.lib.okayama-u.ac.jp/metadata/18090 1968-04-30 http://ousar.lib.okayama-u.ac.jp/metadata/18088 1. On the studies of the relatives of patients with SLE, a family was found in which two sisters were affected with SLE and other two showed positive tests for ANF. In another family, two were positive for ANF, other two families had one positive to ANF. In summery the number being positive for ANF was six (four females and two males). 2. It was emphasized on the basis of the results of various clinical and experimental conditions, that not only genetic factors but also functional unbalance among the genital glands, adrenortex and antibody-producing tissues participated in the onset of autoimmune diseases, especially SLE. 3. Serum γ and α(2) globulin levels were high in majority of SLE. In the course of treatment, there were two cases in which hypergammaglobulinemia became normal and beta-globulin was increased. This hyper-beta-globulinemia may indicate the increased complemental compoments in the serum. 4. All of clinically diagnosed 11 cases of SLE showed a positive LE cell test. The two-hour clotting method was the most sensitive and the cover-glass method followed. The LE factor had been shown to be 7S and 19S globulins by the direct fluorescent antibody technique. 5. ANF was positive in all cases of SLE, even those in remission. The most cases were showed to be heterogenous in all three immunoglobulin classes. IgG ANF was the highest in the titer and persisted for a long period though its titer gradually became low. On the contrary, IgM or IgA ANF (especially the latter) gradually diminished during remission. There were a poor correlation between fluorescent patterns and the types of nuclear antigens. 宮脇,昌二 http://ousar.lib.okayama-u.ac.jp/metadata/18088 1968-04-30 http://ousar.lib.okayama-u.ac.jp/metadata/18087 Fatty acids extracted from Heinz bodies were aided to Ehrlich ascites tumor cells and HeLa cells in tissue culture at various final concentrations of 0.2, 0.1, 0.05, 0.025%, and their effects on these cultured cells were morphologically observed. It was shown that fatty acids derived from Heinz bodies exerted as nearly a potent cytotoxicity as OX substance oa the cultured tumor cells. At the concentration of 0.2 and 0.1%, immediately following thier addition, almost all the cells showed marked degenerative changes such as bubbling and blister formation, lipid granulation in the cytoplasm and pyknosis of the nuclei and this was soon followed by cell floating and death. And even at the lower concentration of 0.05 and 0.025% the cell degeneration was observed in 3 hours and 6 hours respectively and became floating within 24 hours. From these results, it is considered that the formation of Heinz bodies may be a biological reaction as the body defense mechanism, 平木,祥夫 http://ousar.lib.okayama-u.ac.jp/metadata/18087 1968-04-30 http://ousar.lib.okayama-u.ac.jp/metadata/18085 Heinz bodies produced in vitro by X-irradiation or hydroxylamine hydrochloride were separated and collected. The lipids extracted from these Heinz bodies were analyzed as to their lipid and fatty acid composition by thin layer chromatography and gas-liquid chromatography. The purpose of the present experiment is to compare the composition of lipids and fatty acids in the Heinz bodies produced by the two different methods. In addition, the membranes of the untreated red cells were similarly treated and analyzed for comparison. 1. It was found that there were differences in the composition of lipids and fatty acids derived from Heinz bodies under different circumstances in which they were formed, even though they might appear morphologically identical on stained preparations. Namely, Heinz bodies contained a lipid fraction whose chemical composition was not necessarily the same and it was suggested that they were the degenerative products having the same characteristics. 2. Analysis of lipids of Heinz bodies indicated a marked decrease of the phosphatidyl ethanolamine ratio in comparison with the lipid fraction of the normal red cell membranes. 3. With respect to the fatty acid composition of Heinz bodies, there were a decrease of the stearic acid ratio and an increase of the oleic and linoleic acid ratios, resulting in a marked increase of the total unsaturated fatty acid ratio. 4. The development of X-irradiation anemia is due not only to an impaired erythropoiesis in the hematopoietic tissue but to an accelerated destruction of red cells in the peripheral blood. It is postulated that the peripheral red cells are rendered fragile by X-irradiation through an alteration of the lipid composition ratio of the cell membrane. 平木,祥夫 http://ousar.lib.okayama-u.ac.jp/metadata/18085 1968-04-30 http://ousar.lib.okayama-u.ac.jp/metadata/18083 Male Donryu rats were fed with 3 methyl diaminoazobenzen (DAB). The liver was homogenized and incubated with (32)P. phosphatidic acid at 20, 40, 60, 80 and 100 th day. The distributions of (32)P freed from phosphatidic acid by phosphatidic acid phosphatase were studied on lysolecithin, sphingomyelin, phosphatidylcholin and phosphatidylethanolamin. The distribution for lysolecithin and sphingomyelin were no differences between normal and DAB-feeding rat liver. The distribution for phosphatidylcholin was elevated at 80 th day of DAB-feeding rat liver than the normal one, and the distribution for phosphatidyl ethanolamin was bighn in DAB-feeding rat liver then the normal one at 20 th day, but on the controry, it was changed at 40 and 60 th day, and increased in normal than in DAB-feeding rat liver. 吉川,恵 http://ousar.lib.okayama-u.ac.jp/metadata/18083 1968-04-30